DIPLOMA IN MEDICAL LAB TECHNICIAN
DIPLOMA IN MEDICAL LAB TECHNICIAN -- 106
DURATION : 2 YEARS
First Year -- course
Topics :-
1-Anaotomy, Physiology, Clinical Pathology, Genral Pathology
2 -Hematology, Biochemistry-I, Microbiology-I
Second Year -- course
Topics :-
1- Histopathology, Cytopathology, Microbiology-II
2- Blood Banking, Biochemistry-II
Anaotomy
1. Definitions of relevant organ/system. , 2. Cell structure and function. 3. Cellular activities and reproduction. 4. Surface anatomy & Surface marking of major arteries
And veins and related bony structures 5. Outline of Endocrine system.
6. Outline of Skeletal system. 7. Outline of Cardio-vascular system. 8. Outline of lymphatic system. 9. Outline of Respiratory system. 10. Outline of Gastrointestinal system. 11. Special attention towards liver and pancreas. 12. Outline of urogenital system. 13. Outline of central nervous system.
PRACTICAL --Topics
1. To study the gross anatomy of upper extremities. 2. To study the gross anatomy of upper extremities. 3. To study the gross anatomy of Lower extremities. 4. To study the gross anatomy of Lower extremities. 5. To study the gross anatomy of head- neck region. 6. To study the gross anatomy of vertebral column. 7. To study the gross anatomy of cardio vascular system. 8. To study the gross anatomy of cardio vascular system. 9. To study the gross anatomy of spleen & lymph nodes. 1
10. To study the gross anatomy of Respiratory system. 11. To study the gross anatomy of Digestive system. 12. To study the gross anatomy of Endocrine system.
13. To study the gross anatomy of Urinary system. 14. To study the gross anatomy of Genital system. 15. Micro anatomical studies, 16. Micro anatomical studies with the help of Models, 17. Micro anatomical studies Charts & Slides.
PHYSIOLOGY
1. Introduction to Physiology. 2. Physiology of body cells, tissues, organs system. 3. Blood, its components and functions. 4. Blood groups, blood clotting factors, clotting time bleeding time P.T. /P.C. , 5. Blood pressure & Pulse-Definition, measurement techniques, and factors controlling blood pressure. 6. Functions of respiratory system. 7. Functions of Digestive organs. 8. Functions of excretory organs. 9. Fluid and electrolyte balance. 10. Functions of pancreas, Thyroid & parathyroid glands. 11. Introduction to male and female reproductive system. 12. Female and Male reproductive system-Hormonal action. 13. Plasma proteins-Total plasma protein, Albumin, Globulin and Fibrinogen normal values and functions of plasma protein. 14. Hemoglobin-normal values in male, female and children & function of Hemoglobin. 15. Red Blood Cell, normal value, Anemia and Polycythemia. 16. Platelets-Normal value and functions of platelets.
Practical topics:-
1. Study of compound microscope. 2. Collection of Blood sample & commonly used anticoagulants. 3. Preparation of peripheral blood smear. 4. Determination of total leucocyte Count (TLC). 5. Determination of differential leucocyte count (DLC). 6. Determination of total erythrocyte count (RBC). 7. Estimation of Hemoglobin (Sahli’s and other methods) , 8. Determination of bleeding time and clotting time. 9. Determination of Blood groups (A, B, O and Rh system) , 10. Determination of Platelets count., 11. Determination of Arneth count.
12. Measurement of Blood pressure. 13. Examination of respiratory system (Respiratory Rate) 14. Measurement of Heart rate. 15. General Examination.
Pathology
HAEMATOLOGY & CLINICAL PATHOLOGY Topics
1. Introduction to pathology. 2. Composition of blood -1.(RBC,WBC,Platelet) ,3. Composition of blood -2. (Plasma & Plasma Protein) ,4. Routine Instruments in haematology ,5. Collection and Preservation of Blood.,6. Use of autoanalyser in haematology. 7. Making of stains in haematology. 8. Preparation of thick & thin smears.
9. Leishman stain (PPreparation & method of staining) , 10. Other stains in haematology (Preparation & Method of staining).,11. Anti coagulant vials-their preparation and use.
12. Erythocytes & abnormal erythrocytes ,13. Reticulocyte count. ,14. Platelet count.
15. Absolute Values., 16. Hemoparasites , 17. ESR,PCV , 18. Osmotic fragility Test.
19. LE Cell,20. Coagulation Disorders. 21. Lab Diagnosis of Bleeding Disorders. 22. Formation & Composition of Urine , 23. Collection & Preservation of Urine. , 24. Abnormal constituents of urine., 25. Urinometer & Esbach’s Albuminometer , 26. Physical & Chemical examination of urine., 27. Microscopic examination of urine. 28. Liver function test. , 29. Renal Function Test., 30. Examination of body fluids -. 1(Pleural,Peritoneal & Synovial.),31. Examination of body fluids -2.CSF ,32. Semen Examination., 33. Investigations for Aneamia., 34. Hemolytic Aneamia, Foetal Hb.
35. Bone Marrow indications,contra indications & aspiration.
36. Introduction to leukemia , 37. Chronic leukemia & acute leukemia,
38. Use of auto analyser in Haematology.
Practical:--
1. Making of slide and staining. 2. Asessing hemoglobin with different methods. 3. Loading of Neubauer’s chamber. 4. TLC 1 5. DLC , 6. ESR & PCV ,7. Reticulocyte count , 8. RBC Count , 9. Platelet Count , 10. Buffy coat preparation, 11. Coomb’s Test - Direct & Indirect , 12. LE Cell , 13. Osmotic fragility Test , 14. PT/PC , 15. Blood grouping methods , 16. Uses of anti-coagulants , 17. Bone Marrow Aspirations ,18. Cell Count in Acute Leukemia, 19. Cell Count in Chronic Leukemia , 20. Examination of Malarial Parasite. , 21. Examination of Microfillaria. , 22. Fetal Hemoglobin , 23. Urine collection and preservation , 24. 24 hrs. Urine protein estimation , 25. Urine examination – Physical / Chemical , 26. Urine examination – Microscopy , 27. CSF examination. , 28. Semen examination , 29. Other body fluid examination , 30. Rh antibody titre , 31. Automation in haematology .
Bio-Chemistry -1st year
Topics:-
1. Introduction of Biochemistry , 2. Biochemistry Use in Medicine , 3. Units of Measurement , 4. Measurement of Volumetric Apparatus (Pipettes, Flasks & Cylinders) 5. Laboratory Hazards , 6. Laboratory Safety , 7. Laboratory Design & Administration ,
8. Sample Collection , 9. Universal Precautions , 10. Waste Disposal & Management
11. Concept and Calculations Molecular Weight, 12. Concept and Calculations Equivalent Weight, 13. Basic Principles of Centrifugation , 14. Mole, Molar, Buffer & Normal Solution , 15. Definitions of Acid Base , 16. Calorimeter , 17. Preparation of Anticoagulants , 18. Preservation of Anticoagulants , 19. pH & Buffer , 20. Water Purification , 21. Sterilization .
PRACTICAL SCHEDULE
Preparation of Various Types of Solution
1. Normal & Molar , 2. Percentage , 3. Buffers - General Reactions of Carbohydrate , 4. Glucose --General Reactions of Protein , 5. Albumin ,
6 --Examination of Normal Urine --
Physical Examination & Chemical Examination (Chloride, Sulphate, Urea, Ammonia, Phosphate)
7 - Examination of Abnormal Urine --- Physical Examination & Chemical Examination (Protein, Glucose, Ketone Bodies, Bile Salt, Bile Pigment, Blood, Urobilinogen, Chyle, Phenyl Ketonuria, Alkeptonuria) , 8- Estimation of Blood Sugar --- Normal Value & Hyper Value & Hypo Value , 9 - Glucose Tolerance Test -- Normal Value & Hyper Value , 10 - Estimation of Blood Urea -- Normal Value & Hyper Value & Hypo Value ,
11-- Demonstration of Fully Automatic Analyzer -- . Programming of Different Analytes
& Standardization
Microbiology -1st years
Topic:-
1. General introduction & terms used in Microbiology , 2. Safety measures in Microbiology , 3. Universal precautions , 4. Bio-Waste Disposal , 5. Growth & nutrition of Bacteria , 6. Care and Handling of Microscopes , 7. Use, Care and maintenance of common Lab equipments like centrifuges-I , 8. Use, Care and maintenance of common Lab equipments like centrifuges-II , 9. Principles & methods of sterilization , 10. Antiseptics and disinfectants , 11. PH, Buffer & reagents-I , 12. PH, Buffer & reagents-II , 13. Routine bacteria Culture media-I , 14. Routine bacteria Culture media-II , 15. Media for bacterial identification-I , 16. Media for bacterial identification-II , 17. Media for Drug Sensitivity Testing , 18. General characteristics & Classification of Bacteria ,
19. Classification of staining methods smear preparation , 20. Gram stains and other routine stains in Microbiology, 21. Z.N. Stains and other stains for Mycobacterium ,
22. Leishman staining ,23. Gram positive and negative Cocci , 24. Gram negative bacilli
25. Gram positive bacilli , 26. Anaerobic bacteria , 27. Mechanism of drug resistance in bacteria . , 28. Anti bacterial sensitivity testing-I , 29. Anti bacterial sensitivity testing-II
PRACTICAL -- Topics
1. Microscopy , 2. Preparation of load for autoclaving & hot air sterilization ,3. Autoclaving , 4. Use of hot air oven , 5. Disinfection , 6. Preparation of Buffer & reagents 7. Preparation of Culture media (Selective medias) , 8. Preparation of Culture media (Special medias) , 9. Smear preparation , 10. Use of centrifuges , 11. Preparation of stains , 12. Gram’s staining , 13. Zeihl Neelsen staining , 14. Leishman / romanowsky staining , 15. Albert’s & other special staining , 16. Inoculation of culture media-I , 17. Inoculation of culture media-II , 18. Drug Sensitivity Testing-I , 19. Drug Sensitivity Testing-II .
Paper 1st Year
|
Paper |
Subject |
Marks |
Duration |
|
|
Paper-l |
Anatomy, Physiology |
75 |
100 |
3hrs |
|
Internal Assessment |
25 |
|||
|
Paper-ll |
Hematology ,Clinical & General Pathology |
75 |
100 |
3hrs |
|
Internal Assessment |
25 |
|||
|
Paper-lll |
Biochemistry-I, Microbiology-I |
75 |
100 |
3hrs |
|
Internal Assessment |
25 |
Practical - 1st year
|
Paper |
Subject |
Marks |
Duration |
|
Paper-l |
Anatomy, Physiology , Hematology, |
100 |
3hrs |
|
Paper-l l |
Clinical Pathology, General Pathology , Biochemistry-I, Microbiology-I |
100 |
3hrs |
SECOND YEAR –COURSE
Second Year --
HISTOPATHOLOGY & CYTOLOGY
Topics ;-
1. Instruments in Histopathology lab – 1. For grossing & for procesing., 2. Instruments in Histopathology lab – 2. For section cutting & staining. 3. Receiving of sample in Histopathology , 4. Registration of samples and record keeping , 5. Preservation of samples in Histopathology. 6. Grossing of general pathology specimens. , 7. Grossing of respiratory system , 8. Grossing of GIT , 9. Grossing of Hepatobiliary system 10. Grossing of male gential system ,11. Grossing of female genital system , 12. Grossing of breast tissue. 13. Grossing of Urinary system , 14. Grossing of Bones , 15. Grossing of thyroid and and endocrine glands , 16. Grossing of Brain tissue , 17. Tissue Blocking and section cutting. , 18. Reagents in Histopathology. , 19. Staining of slides in Histopathology I (H & E ). , 20. Staining of slides in Histopathology II(Retic /PAS/VG/Amyloid). 21. Paraffin blocks filing. 22. Slide filing in Histopathology , 23. Specimen mounting & Labeling., 24. Cataloguing for museum , 25. Instruments in Cytopathology laboratory. , 26. Receiving of samples in Cytopathology , 27. Preservatives used in Cytopathology , 28. Staining of slides in cytopathology-1: H & E. 29. Staining of slides in cytopathology -2:Pap / gimsa ,&a